The Nuwayrat Individual | Whole-Genome Ancestry Of An Old Kingdom Egyptian

Nuerat Man

Ancient Egyptian society was one of the most influential and long-lasting civilizations in history, thriving for thousands of years. It reached its height during what is known as the Dynastic Period, which spanned from about 3150 to 30 BCE. Despite its rich history, there has been a challenge in studying the genetic makeup of ancient Egyptians due to poor preservation of DNA over time. Previous efforts to understand how various regions of the world, particularly around Egypt, were interrelated have been impeded because scientists have been unable to sequence their entire genomes—until now.

Recently, researchers successfully sequenced the whole genome of an adult male whose remains were unearthed from a site called Nuwayrat, located in Egypt. He lived approximately between 2855 and 2570 BCE, just after the unification of Upper and Lower Egypt. His remains were found in a specially constructed ceramic pot within a rock-cut tomb, a method that may have helped in preserving his DNA better than other burial techniques used at the time.

Genetic Findings

The analysis of his genome revealed that most of his genetic ancestry came from North African populations, particularly those from the Neolithic era, a time when humans transitioned from nomadic hunter-gatherers to settled agricultural communities. Interestingly, about 20% of his genetic lineage was linked to populations from the eastern Fertile Crescent, including areas of present-day Mesopotamia. This finding suggests that there was a form of interaction or connection between these regions beyond mere trade of goods or cultural artifacts, indicating potential human migration.

Fig. 1
a, Geographic location of the Nuwayrat cemetery (red dot) and the previously sequenced Third Intermediate Period individuals from Abusir el-Meleq20 (purple diamond). b, Pottery vessel in which the Nuwayrat individual was discovered. c, Cervical vertebrae belonging to the Nuwayrat individual with evidence of extreme osteoarthritis (arrows). d, Summary of genomic and radiocarbon data. See the detailed breakdown of the quality indicators and calibration results for the three replicates and the combined date in Supplementary Table 2e, Egyptian civilization timeline and radiocarbon date of the Nuwayrat and Third Intermediate Period individuals. mtDNA, mitochondrial DNA. Photo in b reproduced courtesy of the Garstang Museum of Archaeology, University of Liverpool.

Scientists have long believed that the Egyptians were mainly a local population with minimal foreign influences. However, this new genetic evidence indicates that early Egyptians interacted and mixed with neighboring populations in other regions, particularly during critical phases of development such as the Neolithic and Bronze Age, when agricultural practices and writing systems began to emerge.

Cultural Exchange and Migration

The study dives deeper into cultural exchanges between Egypt and neighboring areas, noting that these connections weren’t just limited to the circulation of objects like plants and animals but also involved the movement of people, which contributed to genetic diversity. This adds another layer to our understanding of how ancient civilizations influenced one another over time.

Historical Context

The Egyptian dynastic civilization, known for its monumental architecture—like the pyramids—and advancements in technology, flourished after the political unification of Egypt. This period marked significant achievements, including the construction of the Great Pyramid of Giza, built for the Pharaoh Khufu.

Research over the past few decades, particularly bioarchaeological studies—which focus on human remains—has aimed to paint a clearer picture of the ancient Egyptians, especially concerning their relationships with other populations in North Africa and West Asia. However, without adequate ancient genomic data, understanding continuity and change in the population remained elusive.

Multiple historical events could have affected the genetic landscape of ancient Egyptians, including wars, invasions by foreign rulers, and times of political upheaval, all of which could have shifted the genetic makeup of the populace.

Fig. 3
a, Ancestry proportion of Nuwayrat and comparative Bronze Age Levantine and Anatolian genomes for the best-fit full model (qpAdm). Alternative same-rank models passing P > 0.05 with a lower P value are shown in Supplementary Table 6. Values represent best-fitting model estimates ± 1 standard error. This analysis was conducted over n = 537,543 SNPs for NUE001, n = 518,994 SNPs for Anatolia_BA, n = 554,622 SNPs for Ain’ Ghazal, n = 493,274 SNPs for Ashkelon, n = 578,969 SNPs for Baq’ah, n = 574,452 SNPs for Ebla, n = 552,505 SNPs for Hazor and n = 513,561 SNPs for Tel Shaddud. b, Estimation of the best source responsible for deviation of the Nuwayrat genome from the Middle Neolithic Morocco group as f4(NUE001, Morocco_MN; Mesopotamia_N, Juǀʼhoan). Symbols represent f4 value ± 1 standard error. *Z score > 2; **Z score > 3. The analysis was conducted over 280,544 SNPs. c,d, Map (c) and timeline (d) of rotating sources used to infer the proximal ancestry of the Nuwayrat and Bronze Age Levantine and Anatolian genomes (shown in a), with the dark-yellow area corresponding to the Fertile Crescent. The timeline in d is based on Egyptian cultural transition dates.

The Significance of the Nuwayrat Individual

The remains from Nuwayrat provide critical insights into early Egyptian society. Not only does this individual help trace the genetic lineage back to North African groups and those in the eastern Fertile Crescent, but he also serves as a reminder of the potential relationships and exchanges that took place in the ancient world. Although his burial in a high-status tomb typical for elite individuals might not represent the genetics of the broader population, it opens avenues for future research into ancient Egyptian lineage.

Diet,  Phenotypic Traits, and Lifestyle

Analyzing features of his skeleton has allowed researchers to infer aspects of his life, including physical appearance, health status, and even aspects of his diet. Based on findings from his bones, he had brown eyes, brown hair, and skin ranging from dark to black. Age estimation through skeletal analysis suggested that he lived to be around 44 to 64 years old, which was considered relatively advanced for his time.

The analysis also indicates that he likely had a diet typical of ancient Egyptians, composed of land-based protein sources and plants like wheat and barley. These dietary habits reveal much about the lifestyle and resource access of Egyptians during the Early Dynastic and Old Kingdom periods.

Evidence of Genetic Admixture

By using advanced statistical models to determine ancestry components, researchers can trace various genetic influences back and see how populations mix over time. The Nuwayrat genome provided a wealth of data that shows substantial ties to earlier North African populations and highlighted genetic links to populations in Mesopotamia. This evidence reinforces the idea that Egypt was involved in a broader web of cultural and demographic exchanges during significant historical moments, particularly as agriculture and complex societies began to form.

Researchers emphasize that, while these findings come from a single ancient individual, they reflect broader trends observed in the wider population. The genetic analysis sheds light on the history of how ancient Egypt interacted with other cultures and communities, all within the context of their environments.

The Nuwayrat individual
Facial reconstruction of the Nuwayrat individual by Andrew Olatunde-Adetitun/The Kings Monologue

Conclusion

The Nuwayrat genome deepens our understanding of ancient Egypt, depicting a society that was dynamically connected to its neighbors. Research into this ancient individual’s DNA unravels stories of migration, interconnectivity, and cultural exchange that shaped the modern inhabitants of the region. The exploration of ancient DNA not only enriches our knowledge of past civilizations but also sets a foundation for future inquiries into the complex tapestry of human history and ancestry.

Source: Whole-genome ancestry of an Old Kingdom Egyptian

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